KMID : 0545120140240091207
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Journal of Microbiology and Biotechnology 2014 Volume.24 No. 9 p.1207 ~ p.1215
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GST2 is Required for Nitrogen Starvation-Induced Filamentous Growth in Candida albicans
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Lee So-Hyoung
Chung Soon-Chun Shin Jong-Heon Oh Ki-Bong
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Abstract
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Candida albicans, the major human fungal pathogen, undergoes morphological transition from the budding yeast form to filamentous growth in response to nitrogen starvation. In this study, we identified a new function of GST2, whose expression was required for filamentous growth of C. albicans under nitrogen-limiting conditions. The Gst2p showed Gst activity and required response to oxidative stress. The ¥Ägst2 mutant displayed predominantly yeast phase growth in low ammonium media. Such morphological defect of ¥Ägst2 mutants was not rescued by overexpression of Mep2p, Cph1p, or Efg1p, but was rescued by either overexpression of a hyperactive RAS1G13V allele or through exogenous addition of cyclic AMP. In addition, the ¥Ägst2 mutants had lower levels of RAS1 transcripts than wild-type cells under conditions of nitrogen starvation. These results were consistent with the Ras1-cAMP pathway as a possible downstream target of Gst2p. These findings suggest that Gst2p is a significant component of nitrogen starvation-induced filamentation in C. albicans.
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KEYWORD
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Candida albicans, nitrogen starvation, GST2, morphogenesis, filamentation, signaling pathway
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